metschnikowiaceae cah: functional enrichment (novelty)
One-sided hypergeometric test per term; Benjamini-Hochberg FDR within each scope, level and vocabulary. Candidates are the novelty candidates: gene families present in the IN group and absent from the OUT group. Universe: proteome. Background clustered: True (49255 sequences in 11312 clusters, mmseqs easy-cluster --min-seq-id 0.3 -c 0.8). Family level: one unit per candidate family and per background cluster.
Sources: Pfam: 2026-01-27-Pfam38.2; InterPro entries: 110.0; GO: releases/2026-07-26; analysis generated 2026-10-12T18:41:36. Source tables: studies/fungi/metschnikowiaceae_cah/analysis/enrichment/outputs/proteome_clustered.
Full record: PROVENANCE. Run report: report.html.
Caveats
- Annotation provenance is not fully recorded: PROVENANCE.yaml lists the per-species annotation files and their checksums, but not the UniProt release they come from.
- GO results are secondary. GO annotations include computational evidence (IEA, IBA); the GO evidence column shows if a term has non-IEA support.
- Candidate families (pipeline clustering of candidates) and background clusters (mmseqs clustering of the background proteome) are made by different runs, so family-level counts are not fully comparable.
- Low annotation: the candidate set has less than 50% annotation for Pfam 8.7%, InterPro 18.4%, GO 5.3%, any vocabulary 18.4%. Enrichment for these vocabularies has low power.
Summary figure
Annotation coverage
Fraction of proteins with at least one term per vocabulary. Universe = all proteins of the species; candidates = the candidate set.
| Species | Set | Proteins | Pfam | Pfam clan | InterPro | GO | Any |
|---|---|---|---|---|---|---|---|
| Candauri | universe | 5433 | 86.7% | 68.6% | 89.7% | 79.6% | 90.0% |
| Candauri | candidates | 18 | 0.0% | 0.0% | 5.6% | 0.0% | 5.6% |
| Candhaem | universe | 5240 | 85.7% | 68.8% | 89.0% | 80.3% | 89.3% |
| Candhaem | candidates | 19 | 10.5% | 5.3% | 31.6% | 15.8% | 31.6% |
| Candpseu | universe | 5132 | 85.3% | 68.6% | 88.4% | 80.8% | 88.8% |
| Candpseu | candidates | 23 | 8.7% | 0.0% | 21.7% | 8.7% | 21.7% |
| Candduob | universe | 5170 | 85.5% | 68.5% | 88.9% | 80.5% | 89.1% |
| Candduob | candidates | 23 | 8.7% | 4.3% | 8.7% | 4.3% | 8.7% |
| Clavlusi | universe | 5932 | 79.7% | 63.0% | 83.0% | 74.8% | 83.3% |
| Clavlusi | candidates | 21 | 0.0% | 0.0% | 9.5% | 0.0% | 9.5% |
| Sunginte | universe | 5921 | 84.5% | 66.9% | 88.0% | 78.6% | 88.4% |
| Sunginte | candidates | 16 | 0.0% | 0.0% | 18.8% | 0.0% | 18.8% |
| Metsbicu | universe | 5826 | 79.2% | 61.3% | 82.6% | 74.5% | 82.9% |
| Metsbicu | candidates | 31 | 9.7% | 3.2% | 12.9% | 6.5% | 12.9% |
| Metsaff | universe | 5795 | 81.3% | 64.1% | 84.8% | 75.5% | 85.1% |
| Metsaff | candidates | 30 | 10.0% | 3.3% | 26.7% | 6.7% | 26.7% |
| Austsaop | universe | 5012 | 86.6% | 68.2% | 90.3% | 81.4% | 90.5% |
| Austsaop | candidates | 25 | 24.0% | 4.0% | 28.0% | 4.0% | 28.0% |
| ALL_IN | universe | 49461 | 83.7% | 66.3% | 87.0% | 78.3% | 87.4% |
| ALL_IN | candidates | 206 | 8.7% | 2.4% | 18.4% | 5.3% | 18.4% |
Tests summary
Per vocabulary: terms with FDR < 0.05 / terms tested. Protein level counts proteins; family level counts candidate families and background clusters.
| Scope | Level | Cand. units | Pfam sig / tested | Pfam clan sig / tested | InterPro sig / tested | GO sig / tested |
|---|---|---|---|---|---|---|
| whole_set | family | 129 | 0 / 511 | 0 / 254 | 1 / 1149 | 0 / 3074 |
| whole_set | protein | 206 | 3 / 3873 | 0 / 460 | 6 / 8116 | 7 / 5935 |
| Austsaop | family | 25 | 0 / 151 | 0 / 178 | 0 / 443 | 0 / 1869 |
| Austsaop | protein | 25 | 0 / 188 | 0 / 192 | 0 / 534 | 0 / 1968 |
| Candauri | family | 18 | 0 / 0 (skipped_no_annotated_candidates) | 0 / 0 (skipped_no_annotated_candidates) | 0 / 459 | 0 / 0 (skipped_no_annotated_candidates) |
| Candauri | protein | 18 | 0 / 0 (skipped_no_annotated_candidates) | 0 / 0 (skipped_no_annotated_candidates) | 0 / 576 | 0 / 0 (skipped_no_annotated_candidates) |
| Candduob | family | 23 | 0 / 164 | 0 / 179 | 0 / 467 | 0 / 1938 |
| Candduob | protein | 23 | 0 / 208 | 0 / 192 | 0 / 571 | 0 / 2056 |
| Candhaem | family | 19 | 0 / 163 | 0 / 183 | 0 / 471 | 0 / 1954 |
| Candhaem | protein | 19 | 0 / 218 | 0 / 195 | 0 / 594 | 0 / 2077 |
| Candpseu | family | 23 | 0 / 165 | 0 / 0 (skipped_no_annotated_candidates) | 0 / 475 | 0 / 2306 |
| Candpseu | protein | 23 | 0 / 209 | 0 / 0 (skipped_no_annotated_candidates) | 0 / 582 | 0 / 2418 |
| Clavlusi | family | 21 | 0 / 0 (skipped_no_annotated_candidates) | 0 / 0 (skipped_no_annotated_candidates) | 0 / 485 | 0 / 0 (skipped_no_annotated_candidates) |
| Clavlusi | protein | 21 | 0 / 0 (skipped_no_annotated_candidates) | 0 / 0 (skipped_no_annotated_candidates) | 0 / 590 | 0 / 0 (skipped_no_annotated_candidates) |
| Metsaff | family | 30 | 0 / 152 | 0 / 178 | 0 / 459 | 0 / 1885 |
| Metsaff | protein | 30 | 0 / 212 | 0 / 191 | 0 / 592 | 0 / 2001 |
| Metsbicu | family | 31 | 0 / 143 | 0 / 174 | 0 / 424 | 0 / 2400 |
| Metsbicu | protein | 31 | 0 / 195 | 0 / 186 | 0 / 550 | 0 / 2507 |
| Sunginte | family | 16 | 0 / 0 (skipped_no_annotated_candidates) | 0 / 0 (skipped_no_annotated_candidates) | 1 / 487 | 0 / 0 (skipped_no_annotated_candidates) |
| Sunginte | protein | 16 | 0 / 0 (skipped_no_annotated_candidates) | 0 / 0 (skipped_no_annotated_candidates) | 1 / 629 | 0 / 0 (skipped_no_annotated_candidates) |
Top 25 Pfam terms (whole set, family level)
25 rows shown, ordered by FDR then p-value; 0 have FDR < 0.05 (bold).
| Term | Name | k / N cand. | n / M bg | Fold | FDR (BH) |
|---|---|---|---|---|---|
| PF05436 | MF_alpha_N: Mating factor alpha precursor N-terminus | 1 / 12 | 5 / 7337 | 122.283 | 1.000 |
| PF08303 | tRNA_lig_kinase: tRNA ligase kinase domain | 1 / 12 | 5 / 7337 | 122.283 | 1.000 |
| PF06687 | SUR7: SUR7/PalI family | 1 / 12 | 9 / 7337 | 67.935 | 1.000 |
| PF00018 | SH3_1: SH3 domain | 1 / 12 | 34 / 7337 | 17.983 | 1.000 |
| PF11765 | Hyphal_reg_CWP: Hyphally regulated cell wall protein N-terminal | 1 / 12 | 47 / 7337 | 13.009 | 1.000 |
| PF00004 | AAA: ATPase family associated with various cellular activities (AAA) | 0 / 12 | 36 / 7337 | 0.0 | 1.000 |
| PF00005 | ABC_tran: ABC transporter | 0 / 12 | 45 / 7337 | 0.0 | 1.000 |
| PF00006 | ATP-synt_ab: ATP synthase alpha/beta family, nucleotide-binding domain | 0 / 12 | 6 / 7337 | 0.0 | 1.000 |
| PF00009 | GTP_EFTU: Elongation factor Tu GTP binding domain | 0 / 12 | 15 / 7337 | 0.0 | 1.000 |
| PF00010 | HLH: Helix-loop-helix DNA-binding domain | 0 / 12 | 19 / 7337 | 0.0 | 1.000 |
| PF00012 | HSP70: Hsp70 protein | 0 / 12 | 7 / 7337 | 0.0 | 1.000 |
| PF00013 | KH_1: KH domain | 0 / 12 | 15 / 7337 | 0.0 | 1.000 |
| PF00022 | Actin: Actin | 0 / 12 | 8 / 7337 | 0.0 | 1.000 |
| PF00023 | Ank: Ankyrin repeat | 0 / 12 | 9 / 7337 | 0.0 | 1.000 |
| PF00025 | Arf: ADP-ribosylation factor family | 0 / 12 | 6 / 7337 | 0.0 | 1.000 |
| PF00026 | Asp: Eukaryotic aspartyl protease | 0 / 12 | 27 / 7337 | 0.0 | 1.000 |
| PF00027 | cNMP_binding: Cyclic nucleotide-binding domain | 0 / 12 | 5 / 7337 | 0.0 | 1.000 |
| PF00043 | GST_C: Glutathione S-transferase, C-terminal domain | 0 / 12 | 5 / 7337 | 0.0 | 1.000 |
| PF00063 | Myosin_head: Myosin head (motor domain) | 0 / 12 | 7 / 7337 | 0.0 | 1.000 |
| PF00067 | p450: Cytochrome P450 | 0 / 12 | 14 / 7337 | 0.0 | 1.000 |
| PF00069 | Pkinase: Protein kinase domain | 0 / 12 | 130 / 7337 | 0.0 | 1.000 |
| PF00071 | Ras: Ras family | 0 / 12 | 21 / 7337 | 0.0 | 1.000 |
| PF00072 | Response_reg: Response regulator receiver domain | 0 / 12 | 12 / 7337 | 0.0 | 1.000 |
| PF00076 | RRM_1: RNA recognition motif | 0 / 12 | 81 / 7337 | 0.0 | 1.000 |
| PF00078 | RVT_1: Reverse transcriptase (RNA-dependent DNA polymerase) | 0 / 12 | 13 / 7337 | 0.0 | 1.000 |
Top 25 InterPro terms (whole set, family level)
25 rows shown, ordered by FDR then p-value; 1 have FDR < 0.05 (bold).
| Term | Name | k / N cand. | n / M bg | Fold | FDR (BH) |
|---|---|---|---|---|---|
| IPR006594 | Conserved_site:LIS1 homology motif | 3 / 26 | 15 / 7743 | 59.562 | 0.017 |
| IPR036465 | Homologous_superfamily:von Willebrand factor A-like domain superfamily | 2 / 26 | 23 / 7743 | 25.896 | 1.000 |
| IPR008675 | Domain:Mating factor alpha precursor, N-terminal | 1 / 26 | 5 / 7743 | 59.562 | 1.000 |
| IPR015966 | Domain:tRNA ligase, kinase domain, fungi | 1 / 26 | 5 / 7743 | 59.562 | 1.000 |
| IPR036885 | Homologous_superfamily:SWIB/MDM2 domain superfamily | 1 / 26 | 6 / 7743 | 49.635 | 1.000 |
| IPR009571 | Family:Membrane protein SUR7/Rim9-like, fungi | 1 / 26 | 9 / 7743 | 33.09 | 1.000 |
| IPR001878 | Domain:Zinc finger, CCHC-type | 1 / 26 | 14 / 7743 | 21.272 | 1.000 |
| IPR013087 | Domain:Zinc finger C2H2-type | 2 / 26 | 112 / 7743 | 5.318 | 1.000 |
| IPR036691 | Homologous_superfamily:Endonuclease/exonuclease/phosphatase superfamily | 1 / 26 | 27 / 7743 | 11.03 | 1.000 |
| IPR001810 | Domain:F-box domain | 1 / 26 | 39 / 7743 | 7.636 | 1.000 |
| IPR001452 | Domain:SH3 domain | 1 / 26 | 40 / 7743 | 7.445 | 1.000 |
| IPR036028 | Homologous_superfamily:SH3-like domain superfamily | 1 / 26 | 40 / 7743 | 7.445 | 1.000 |
| IPR021031 | Domain:Hyphally-regulated cell wall protein, N-terminal | 1 / 26 | 47 / 7743 | 6.336 | 1.000 |
| IPR036236 | Homologous_superfamily:Zinc finger C2H2 superfamily | 1 / 26 | 84 / 7743 | 3.545 | 1.000 |
| IPR035979 | Homologous_superfamily:RNA-binding domain superfamily | 1 / 26 | 112 / 7743 | 2.659 | 1.000 |
| IPR012677 | Homologous_superfamily:Nucleotide-binding alpha-beta plait domain superfamily | 1 / 26 | 113 / 7743 | 2.635 | 1.000 |
| IPR001680 | Repeat:WD40 repeat | 1 / 26 | 146 / 7743 | 2.04 | 1.000 |
| IPR036864 | Homologous_superfamily:Zn(2)-C6 fungal-type DNA-binding domain superfamily | 1 / 26 | 155 / 7743 | 1.921 | 1.000 |
| IPR001138 | Domain:Zn(2)Cys(6) fungal-type DNA-binding domain | 1 / 26 | 161 / 7743 | 1.85 | 1.000 |
| IPR036322 | Homologous_superfamily:WD40-repeat-containing domain superfamily | 1 / 26 | 185 / 7743 | 1.61 | 1.000 |
| IPR015943 | Homologous_superfamily:WD40/YVTN repeat-like-containing domain superfamily | 1 / 26 | 195 / 7743 | 1.527 | 1.000 |
| IPR027417 | Homologous_superfamily:P-loop containing nucleoside triphosphate hydrolase | 1 / 26 | 441 / 7743 | 0.675 | 1.000 |
| IPR000008 | Domain:C2 domain | 0 / 26 | 20 / 7743 | 0.0 | 1.000 |
| IPR000073 | Domain:Alpha/beta hydrolase fold-1 | 0 / 26 | 33 / 7743 | 0.0 | 1.000 |
| IPR000086 | Domain:NUDIX hydrolase domain | 0 / 26 | 15 / 7743 | 0.0 | 1.000 |
Top 15 GO terms (whole set, family level) — secondary, computational evidence (IEA/IBA) included
15 rows shown, ordered by FDR then p-value; 0 have FDR < 0.05 (bold).
| Term | Name | k / N cand. | n / M bg | Fold | FDR (BH) | GO evidence | Families with non-IEA |
|---|---|---|---|---|---|---|---|
| GO:0007618 | biological_process:mating | 1 / 8 | 5 / 6807 | 170.175 | 1.000 | IEA_only | 0 |
| GO:0044703 | biological_process:multi-organism reproductive process | 1 / 8 | 5 / 6807 | 170.175 | 1.000 | IEA_only | 0 |
| GO:0003972 | molecular_function:RNA ligase (ATP) activity | 1 / 8 | 6 / 6807 | 141.812 | 1.000 | IEA_only | 0 |
| GO:0008452 | molecular_function:RNA ligase activity | 1 / 8 | 6 / 6807 | 141.812 | 1.000 | IEA_only | 0 |
| GO:0000394 | biological_process:RNA splicing, via endonucleolytic cleavage and ligation | 1 / 8 | 9 / 6807 | 94.542 | 1.000 | IEA_only | 0 |
| GO:0006388 | biological_process:tRNA splicing, via endonucleolytic cleavage and ligation | 1 / 8 | 9 / 6807 | 94.542 | 1.000 | IEA_only | 0 |
| GO:0016886 | molecular_function:ligase activity, forming phosphoric ester bonds | 1 / 8 | 9 / 6807 | 94.542 | 1.000 | IEA_only | 0 |
| GO:0032185 | biological_process:septin cytoskeleton organization | 1 / 8 | 14 / 6807 | 60.777 | 1.000 | IEA_only | 0 |
| GO:0045121 | cellular_component:membrane raft | 1 / 8 | 14 / 6807 | 60.777 | 1.000 | IEA_only | 0 |
| GO:0098857 | cellular_component:membrane microdomain | 1 / 8 | 14 / 6807 | 60.777 | 1.000 | IEA_only | 0 |
| GO:0030865 | biological_process:cortical cytoskeleton organization | 1 / 8 | 25 / 6807 | 34.035 | 1.000 | IEA_only | 0 |
| GO:0030866 | biological_process:cortical actin cytoskeleton organization | 1 / 8 | 25 / 6807 | 34.035 | 1.000 | IEA_only | 0 |
| GO:0030036 | biological_process:actin cytoskeleton organization | 1 / 8 | 42 / 6807 | 20.259 | 1.000 | IEA_only | 0 |
| GO:0030029 | biological_process:actin filament-based process | 1 / 8 | 49 / 6807 | 17.365 | 1.000 | IEA_only | 0 |
| GO:0005938 | cellular_component:cell cortex | 1 / 8 | 53 / 6807 | 16.054 | 1.000 | IEA_only | 0 |
Actin cytoskeleton controls
Known actin-related families and how many of their proteins pass each filter. Protein ID columns are left out.
| control | pfam_ids | desc_regex | IN_universe | IN_in_matrix | IN_pass_ingroup_rule | candidates | near_miss_out1 | near_miss_out2 | n_Candauri | n_Candhaem | n_Candpseu | n_Candduob | n_Clavlusi | n_Sunginte | n_Metsbicu | n_Metsaff | n_Austsaop | n_Candalbi | n_Candtrop | n_Loddelon | n_Meyeguil | n_Debahans | n_Schestip | n_Spatpass | n_Diutrugo | n_Komaphaf | n_Ogatpara | n_Kuracaps | n_Pichkudr | n_Pachtann |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Arp2/3 ARPC2 (P34-Arc) | PF04045 | 9 | 9 | 9 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| Arp2/3 ARPC3 (P21-Arc) | PF04062 | 8 | 8 | 8 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| Arp2/3 ARPC4 | PF05856 | 8 | 8 | 8 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| Arp2/3 ARPC5 (P16-Arc) | PF04699 | 9 | 9 | 9 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| Arp2/3 ARPC1 (description only) | arp2/3 complex.*(41|p41|subunit 1)|ARPC1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| Arp2 / Arp3 (description only) | actin-related protein (2|3)\b|\bARP[23]\b | 50 | 50 | 50 | 0 | 0 | 0 | 6 | 5 | 6 | 7 | 6 | 5 | 4 | 5 | 6 | 7 | 7 | 7 | 7 | 6 | 6 | 7 | 7 | 8 | 7 | 7 | 5 | 7 | |
| WAVE complex NCKAP1 | PF09735 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| WAVE complex CYFIP (FragX_IP) | PF05994 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| WAVE complex ABI | PF07815|PF27594 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| SCAR/WAVE (SCAR_helical) | PF27569 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| WASP family (WH1) | PF00568 | 9 | 9 | 9 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| WH2 motif (WASP, WAVE, others) | PF02205 | 16 | 16 | 16 | 0 | 0 | 0 | 2 | 2 | 2 | 2 | 1 | 3 | 1 | 1 | 2 | 2 | 3 | 3 | 0 | 2 | 2 | 1 | 2 | 2 | 0 | 2 | 2 | 1 | |
| Formin FH2 | PF02181 | 18 | 18 | 18 | 0 | 0 | 0 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | |
| Formin Drf_GBD / Drf_FH3 / Drf_DAD | PF06371|PF06367|PF06345 | 18 | 18 | 18 | 0 | 0 | 0 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 1 | 2 | 1 | 2 | |
| Ena/VASP (VASP_tetra) | PF08776 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| Coronin (description only) | coronin | 7 | 7 | 7 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| Cortactin (HS1_rep) | PF02218 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| Myosin 2 tail (Myosin_tail_1) | PF01576 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| Myosin head (all myosins) | PF00063 | 26 | 26 | 26 | 0 | 0 | 0 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 4 | 3 |
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