gallery / domain / chytrid_vs_mucoromycota / pairwise-diamond / enrichment (novelty)

chytrid vs mucoromycota: functional enrichment (novelty)

One-sided hypergeometric test per term; Benjamini-Hochberg FDR within each scope, level and vocabulary. Candidates are the novelty candidates: gene families present in the IN group and absent from the OUT group. Universe: proteome. Background clustered: True (101239 sequences in 49924 clusters, mmseqs easy-cluster --min-seq-id 0.3 -c 0.8). Family level: one unit per candidate family and per background cluster.

Sources: Pfam: 2026-01-27-Pfam38.2; InterPro entries: 110.0; GO: releases/2026-07-26; analysis generated 2026-10-12T14:46:00. Source tables: studies/fungi/chytrid_vs_mucoromycota/analysis/enrichment/outputs/proteome_clustered. Full record: PROVENANCE. Run report: report.html.

Caveats

Annotation provenance is not fully recorded: PROVENANCE.yaml lists the per-species annotation files and their checksums, but not the UniProt release they come from.
GO results are secondary. GO annotations include computational evidence (IEA, IBA); the GO evidence column shows if a term has non-IEA support.
Candidate families (pipeline clustering of candidates) and background clusters (mmseqs clustering of the background proteome) are made by different runs, so family-level counts are not fully comparable.

Summary figure

Annotation coverage

Fraction of proteins with at least one term per vocabulary. Universe = all proteins of the species; candidates = the candidate set.

Species	Set	Proteins	Pfam	Pfam clan	InterPro	GO	Any
Batrdend	universe	8610	68.0%	55.5%	75.5%	64.4%	75.9%
Batrdend	candidates	483	73.3%	53.2%	85.9%	51.5%	86.3%
Batrsala	universe	11060	60.0%	49.6%	66.2%	53.6%	66.6%
Batrsala	candidates	446	70.2%	44.4%	85.4%	57.4%	85.4%
Spizpunc	universe	8940	77.0%	62.6%	81.7%	68.6%	82.1%
Spizpunc	candidates	442	75.6%	46.8%	85.8%	57.0%	86.4%
Powehirt	universe	6533	88.3%	72.6%	92.2%	79.7%	92.5%
Powehirt	candidates	373	74.0%	45.8%	85.2%	58.2%	85.5%
Geravari	universe	8696	72.9%	59.8%	78.6%	65.8%	79.0%
Geravari	candidates	419	73.3%	47.0%	85.0%	57.3%	85.7%
Syncendo	universe	7895	64.4%	52.3%	69.6%	59.2%	70.0%
Syncendo	candidates	337	69.4%	44.5%	83.4%	59.4%	84.6%
Syncmicr	universe	6304	90.2%	75.6%	94.1%	82.1%	94.3%
Syncmicr	candidates	362	72.9%	47.5%	86.5%	59.7%	87.0%
Chytconf	universe	10661	80.1%	68.1%	86.9%	72.9%	87.3%
Chytconf	candidates	504	75.8%	51.8%	88.7%	62.3%	88.9%
Polystew	universe	9229	72.1%	60.4%	80.4%	63.9%	80.9%
Polystew	candidates	455	69.0%	45.3%	84.4%	54.9%	85.7%
Rhizglob	universe	16725	56.7%	48.2%	65.1%	50.4%	65.5%
Rhizglob	candidates	548	67.7%	48.9%	83.0%	55.5%	83.6%
Rhizrose	universe	11454	62.5%	51.2%	70.1%	55.4%	70.5%
Rhizrose	candidates	499	68.3%	43.1%	82.0%	55.3%	82.6%
ALL_IN	universe	106107	69.8%	57.9%	76.3%	63.0%	76.7%
ALL_IN	candidates	4868	71.7%	47.3%	85.0%	57.0%	85.6%

Tests summary

Per vocabulary: terms with FDR < 0.05 / terms tested. Protein level counts proteins; family level counts candidate families and background clusters.

Scope	Level	Cand. units	Pfam sig / tested	Pfam clan sig / tested	InterPro sig / tested	GO sig / tested
whole_set	family	2151	140 / 2228	35 / 442	251 / 4623	300 / 4450
whole_set	protein	4868	326 / 4739	51 / 513	532 / 9942	407 / 5959
Batrdend	family	410	6 / 238	10 / 200	10 / 629	60 / 1974
Batrdend	protein	483	8 / 307	8 / 215	17 / 785	79 / 2076
Batrsala	family	422	2 / 263	8 / 208	7 / 682	64 / 1943
Batrsala	protein	446	8 / 330	11 / 223	24 / 819	78 / 2038
Chytconf	family	432	6 / 391	8 / 244	13 / 972	89 / 2136
Chytconf	protein	504	11 / 590	8 / 280	20 / 1389	114 / 2408
Geravari	family	414	4 / 315	6 / 228	8 / 793	90 / 2041
Geravari	protein	419	4 / 372	6 / 241	8 / 928	97 / 2142
Polystew	family	417	5 / 315	8 / 215	13 / 799	77 / 1984
Polystew	protein	455	10 / 406	10 / 235	25 / 982	86 / 2095
Powehirt	family	368	4 / 302	6 / 222	7 / 755	70 / 1999
Powehirt	protein	373	4 / 358	10 / 234	8 / 880	75 / 2078
Rhizglob	family	496	4 / 440	9 / 244	16 / 1050	97 / 2440
Rhizglob	protein	548	15 / 596	14 / 268	32 / 1369	123 / 2624
Rhizrose	family	488	6 / 370	8 / 233	11 / 908	91 / 2128
Rhizrose	protein	499	6 / 408	8 / 244	12 / 1016	94 / 2194
Spizpunc	family	438	7 / 360	8 / 231	10 / 882	72 / 2442
Spizpunc	protein	442	7 / 424	8 / 245	10 / 1021	74 / 2536
Syncendo	family	331	1 / 248	3 / 198	4 / 628	70 / 1865
Syncendo	protein	337	2 / 296	6 / 214	5 / 735	72 / 1923
Syncmicr	family	348	4 / 287	8 / 211	6 / 731	70 / 1940
Syncmicr	protein	362	5 / 364	11 / 224	7 / 890	75 / 2049

Top 25 Pfam terms (whole set, family level)

25 rows shown, ordered by FDR then p-value; 25 have FDR < 0.05 (bold).

Term	Name	k / N cand.	n / M bg	Fold	FDR (BH)
PF00211	Guanylate_cyc: Adenylate and Guanylate cyclase catalytic domain	33 / 1459	55 / 29351	12.07	6.79e-26
PF02493	MORN: MORN repeat	20 / 1459	25 / 29351	16.094	3.49e-19
PF14033	DUF4246: Protein of unknown function (DUF4246)	18 / 1459	22 / 29351	16.46	1.39e-17
PF13879	Hmw_CFAP97: Hemingway/CFA97	20 / 1459	30 / 29351	13.411	7.77e-17
PF02156	Glyco_hydro_26: Glycosyl hydrolase family 26	17 / 1459	26 / 29351	13.154	5.74e-14
PF02485	Branch: Core-2/I-Branching enzyme	11 / 1459	11 / 29351	20.117	1.64e-12
PF02434	Fringe: Fringe-like	11 / 1459	13 / 29351	17.022	1.00e-10
PF04488	Gly_transf_sug: Glycosyltransferase sugar-binding region containing DXD motif	20 / 1459	57 / 29351	7.059	4.31e-10
PF05010	TACC_C: Transforming acidic coiled-coil-containing protein (TACC), C-terminal	9 / 1459	9 / 29351	20.117	4.48e-10
PF13516	LRR_6: Leucine Rich repeat	25 / 1459	96 / 29351	5.239	1.17e-09
PF06294	CH_2: CH-like domain in sperm protein	11 / 1459	16 / 29351	13.831	3.10e-09
PF22544	HYDIN_VesB_CFA65-like_Ig: HYDIN/CFA65/VesB-like, Ig-like domain	10 / 1459	13 / 29351	15.475	4.14e-09
PF18027	Pepdidase_M14_N: Cytosolic carboxypeptidase N-terminal domain	9 / 1459	12 / 29351	15.088	5.95e-08
PF11699	CENP-C_C: Mif2/CENP-C like	7 / 1459	7 / 29351	20.117	1.18e-07
PF14580	LRR_9: Leucine-rich repeat	15 / 1459	47 / 29351	6.42	6.45e-07
PF27813	CCDC148: CCDC148 coiled-coil domain	7 / 1459	8 / 29351	17.603	7.88e-07
PF10629	CMI2B-like: Ciliary microtubule inner protein 2B-like	6 / 1459	6 / 29351	20.117	1.20e-06
PF13863	DUF4200: Domain of unknown function (DUF4200)	6 / 1459	6 / 29351	20.117	1.20e-06
PF14646	MYCBPAP: MYCBP-associated protein family	6 / 1459	6 / 29351	20.117	1.20e-06
PF14988	DUF4515: Domain of unknown function (DUF4515)	6 / 1459	6 / 29351	20.117	1.20e-06
PF15479	DUF4639: Domain of unknown function (DUF4639)	6 / 1459	6 / 29351	20.117	1.20e-06
PF15625	CC2D2AN-C2: CC2D2A N-terminal C2 domain	6 / 1459	6 / 29351	20.117	1.20e-06
PF21666	DUF4246_N: Domain of unknown function DUF4246, N-terminal	6 / 1459	6 / 29351	20.117	1.20e-06
PF23277	Ig_Dlec1_1: Deleted in lung and esophageal cancer protein 1 Ig domain	6 / 1459	6 / 29351	20.117	1.20e-06
PF28302	FLJ43738_C: Uncharacterised protein FLJ43738 C-terminal domain	6 / 1459	6 / 29351	20.117	1.20e-06

Top 25 InterPro terms (whole set, family level)

25 rows shown, ordered by FDR then p-value; 25 have FDR < 0.05 (bold).

Term	Name	k / N cand.	n / M bg	Fold	FDR (BH)
IPR000805	Family:Glycoside hydrolase family 26	24 / 1765	29 / 33597	15.753	7.16e-23
IPR001054	Domain:Adenylyl cyclase class-3/4/guanylyl cyclase	33 / 1765	68 / 33597	9.238	4.74e-21
IPR029787	Homologous_superfamily:Nucleotide cyclase	33 / 1765	71 / 33597	8.847	1.83e-20
IPR022790	Domain:Glycosyl hydrolase family 26 domain	23 / 1765	32 / 33597	13.681	6.64e-20
IPR003409	Repeat:MORN repeat	20 / 1765	25 / 33597	15.228	8.82e-19
IPR049192	Domain:Domain of unknown function DUF4246, C-terminal	18 / 1765	22 / 33597	15.574	3.94e-17
IPR029488	Family:Hemingway/CFA97	20 / 1765	30 / 33597	12.69	2.76e-16
IPR025340	Family:Protein of unknown function DUF4246	18 / 1765	24 / 33597	14.276	4.91e-16
IPR038792	Family:CFAP97 domain-containing protein 1/2	16 / 1765	22 / 33597	13.844	8.95e-14
IPR026050	Family:C1GALT1/C1GALT1-specific chaperone 1	13 / 1765	14 / 33597	17.675	1.37e-13
IPR003406	Family:Glycosyl transferase, family 14	11 / 1765	11 / 33597	19.035	3.15e-12
IPR043538	Family:Xylosyltransferase	11 / 1765	11 / 33597	19.035	3.15e-12
IPR039902	Family:Coiled-coil domain-containing protein CCDC148/CCDC112	12 / 1765	14 / 33597	16.316	1.25e-11
IPR050697	Family:Adenylyl/Guanylyl Cyclase Class-3/4	13 / 1765	19 / 33597	13.024	1.48e-10
IPR003378	Domain:Fringe-like, glycosyltransferase domain	11 / 1765	13 / 33597	16.107	1.73e-10
IPR011989	Homologous_superfamily:Armadillo-like helical	71 / 1765	532 / 33597	2.54	1.73e-10
IPR052394	Family:Leucine-rich repeat-containing protein	12 / 1765	16 / 33597	14.276	1.73e-10
IPR007707	Domain:Transforming acidic coiled-coil-containing protein, C-terminal	9 / 1765	9 / 33597	19.035	7.68e-10
IPR007577	Conserved_site:Glycosyltransferase, DXD sugar-binding motif	20 / 1765	57 / 33597	6.679	1.05e-09
IPR010441	Domain:CH-like domain in sperm protein	11 / 1765	16 / 33597	13.087	6.45e-09
IPR053879	Domain:HYDIN/VesB/CFA65-like, Ig-like domain	10 / 1765	13 / 33597	14.642	8.50e-09
IPR033351	Family:Centrosomal protein POC5	8 / 1765	8 / 33597	19.035	1.15e-08
IPR039915	Family:TACC family	8 / 1765	8 / 33597	19.035	1.15e-08
IPR050821	Family:Cytosolic carboxypeptidase	10 / 1765	14 / 33597	13.597	2.48e-08
IPR052111	Family:Spermatogenesis and Ciliary Microtubule-associated Protein	9 / 1765	11 / 33597	15.574	2.76e-08

Top 15 GO terms (whole set, family level) — secondary, computational evidence (IEA/IBA) included

15 rows shown, ordered by FDR then p-value; 15 have FDR < 0.05 (bold).

Term	Name	k / N cand.	n / M bg	Fold	FDR (BH)	GO evidence	Families with non-IEA
GO:0044782	biological_process:cilium organization	125 / 1157	233 / 25791	11.959	4.15e-102	has_non_IEA	29
GO:0120036	biological_process:plasma membrane bounded cell projection organization	125 / 1157	233 / 25791	11.959	4.15e-102	has_non_IEA	29
GO:0030030	biological_process:cell projection organization	125 / 1157	234 / 25791	11.908	5.70e-102	has_non_IEA	29
GO:0005929	cellular_component:cilium	127 / 1157	259 / 25791	10.93	1.43e-97	has_non_IEA	22
GO:0042995	cellular_component:cell projection	127 / 1157	261 / 25791	10.847	3.34e-97	has_non_IEA	22
GO:0120025	cellular_component:plasma membrane bounded cell projection	127 / 1157	261 / 25791	10.847	3.34e-97	has_non_IEA	22
GO:0030031	biological_process:cell projection assembly	111 / 1157	212 / 25791	11.671	2.69e-89	has_non_IEA	26
GO:0060271	biological_process:cilium assembly	111 / 1157	212 / 25791	11.671	2.69e-89	has_non_IEA	26
GO:0120031	biological_process:plasma membrane bounded cell projection assembly	111 / 1157	212 / 25791	11.671	2.69e-89	has_non_IEA	26
GO:0005930	cellular_component:axoneme	89 / 1157	128 / 25791	15.499	3.71e-87	has_non_IEA	18
GO:0007017	biological_process:microtubule-based process	179 / 1157	679 / 25791	5.876	2.47e-85	has_non_IEA	38
GO:0005815	cellular_component:microtubule organizing center	115 / 1157	349 / 25791	7.345	4.30e-65	has_non_IEA	24
GO:0007018	biological_process:microtubule-based movement	109 / 1157	337 / 25791	7.21	1.23e-60	has_non_IEA	27
GO:0070925	biological_process:organelle assembly	119 / 1157	428 / 25791	6.198	2.78e-58	has_non_IEA	28
GO:0042073	biological_process:intraciliary transport	62 / 1157	99 / 25791	13.96	6.96e-56	has_non_IEA	15

Actin cytoskeleton controls

Known actin-related families and how many of their proteins pass each filter. Protein ID columns are left out.

control	pfam_ids	desc_regex	IN_universe	IN_in_matrix	IN_pass_ingroup_rule	candidates	near_miss_out1	n_Batrdend	n_Batrsala	n_Spizpunc	n_Powehirt	n_Geravari	n_Syncendo	n_Syncmicr	n_Chytconf	n_Polystew	n_Rhizglob	n_Rhizrose	n_Mucocirc	n_Rhizdele	n_Lichcory	n_Phycblak	n_Absiglau	n_Syncrace	n_Choacucu	n_Hessvesi	n_Umbevina	n_Mortisab	n_Thamspha
Arp2/3 ARPC2 (P34-Arc)	PF04045		11	11	11	0	0	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	2	1	1	1	1	1	1
Arp2/3 ARPC3 (P21-Arc)	PF04062		11	11	11	0	0	1	1	1	1	1	1	1	1	1	1	1	1	0	1	1	1	1	1	1	1	1	1
Arp2/3 ARPC4	PF05856		11	11	11	0	0	1	1	1	1	1	1	1	1	1	1	1	1	2	1	1	1	1	1	1	1	1	0
Arp2/3 ARPC5 (P16-Arc)	PF04699		12	12	12	0	0	1	1	1	1	1	1	1	2	1	1	1	1	1	0	1	1	1	1	1	1	1	1
Arp2/3 ARPC1 (description only)		arp2/3 complex.*(41\|p41\|subunit 1)\|ARPC1	2	2	2	0	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Arp2 / Arp3 (description only)		actin-related protein (2\|3)\b\|\bARP[23]\b	70	70	69	0	0	6	7	6	7	6	7	6	6	8	5	6	6	7	5	5	8	5	8	7	7	7	4
WAVE complex NCKAP1	PF09735		9	9	9	8	0	1	1	1	1	1	1	1	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0
WAVE complex CYFIP (FragX_IP)	PF05994		9	9	9	9	0	1	1	1	1	1	1	1	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0
WAVE complex ABI	PF07815\|PF27594		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SCAR/WAVE (SCAR_helical)	PF27569		9	9	9	9	0	1	1	1	1	1	1	1	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0
WASP family (WH1)	PF00568		11	11	11	0	0	1	1	1	1	1	1	1	1	1	1	1	3	1	1	1	2	3	2	3	2	2	1
WH2 motif (WASP, WAVE, others)	PF02205		12	10	8	0	2	3	1	1	1	1	1	1	1	1	0	1	2	1	2	0	3	2	2	3	2	3	0
Formin FH2	PF02181		50	50	49	3	0	4	6	4	4	4	3	4	5	6	5	5	8	9	7	3	9	6	6	7	4	4	2
Formin Drf_GBD / Drf_FH3 / Drf_DAD	PF06371\|PF06367\|PF06345		40	40	36	2	1	3	4	4	3	4	2	3	3	4	3	7	8	11	7	6	10	7	8	8	7	7	2
Ena/VASP (VASP_tetra)	PF08776		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Coronin (description only)		coronin	27	27	26	1	0	1	1	3	3	4	1	2	4	2	2	4	1	3	2	2	3	2	1	2	2	2	1
Cortactin (HS1_rep)	PF02218		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Myosin 2 tail (Myosin_tail_1)	PF01576		12	11	11	0	1	1	1	1	1	1	1	1	1	1	1	2	0	0	0	0	0	0	0	0	0	1	1
Myosin head (all myosins)	PF00063		101	101	100	0	0	7	8	9	9	7	6	9	13	7	14	12	17	19	14	16	18	11	16	13	8	9	5

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