generated: '2026-10-24T14:40:05' study: yc_ustilaginomycotina script: path: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/zoosporic_dikarya/analysis/enrichment/enrich_novelties_v2.py sha256: d1e8fe16f6842e86fb6c226f822c8c173450a8fec660ca6879a6f8a821ad5aa7 command: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/.pixi/envs/default/bin/python3 /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/zoosporic_dikarya/analysis/enrichment/enrich_novelties_v2.py --results /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/results/yc_ustilaginomycotina --config /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/config.csv --annotations /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations --study yc_ustilaginomycotina --cache-dir /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/analysis/enrichment/outputs/cache --threads 4 --pfam-dat /srv/projects/db/pfam/2026-01-27-Pfam38.2/Pfam-A.hmm.dat --interpro-entries /srv/projects/db/interproscan/6.0.0/110.0/interpro/110.0/entries.json --controls /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/zoosporic_dikarya/analysis/enrichment/actin_controls.tsv --ingroup-min-frac 0.75 --universe proteome --cluster-background --direction novelty --out-dir /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/analysis/enrichment/outputs/novelty/proteome_clustered class: ephemeral analysis output (not tracked; regenerate with the command above) direction: novelty target_group: label: IN species: - Malaglob - Malasymp - Moesaphi - Kalmbras other_group: label: OUT species: - Mycomayd - Sporreil - Ustihord - Cerabomb universe: proteome annotations: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations annotation_files: Malaglob: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000008837.tsv.gz Malasymp: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000186303.tsv.gz Moesaphi: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000019462.tsv.gz Kalmbras: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000019377.tsv.gz Mycomayd: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000000561.tsv.gz Sporreil: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000008867.tsv.gz Ustihord: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000006174.tsv.gz Cerabomb: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000054845.tsv.gz annotation_sources: Malaglob: not recorded (no sidecar) Malasymp: not recorded (no sidecar) Moesaphi: not recorded (no sidecar) Kalmbras: not recorded (no sidecar) Mycomayd: not recorded (no sidecar) Sporreil: not recorded (no sidecar) Ustihord: not recorded (no sidecar) Cerabomb: not recorded (no sidecar) vocabulary_coverage: pfam: species_with_terms: - Malaglob - Malasymp - Moesaphi - Kalmbras species_without_terms: [] n_with: 4 n_species: 4 pfam_clan: species_with_terms: - Malaglob - Malasymp - Moesaphi - Kalmbras species_without_terms: [] n_with: 4 n_species: 4 interpro: species_with_terms: - Malaglob - Malasymp - Moesaphi - Kalmbras species_without_terms: [] n_with: 4 n_species: 4 go: species_with_terms: - Malaglob - Malasymp - Moesaphi - Kalmbras species_without_terms: [] n_with: 4 n_species: 4 parameters: min_bg: 5 exclude_iea: false report_ids_fdr: 0.05 ingroup_min_frac: 0.75 other_max_frac: 0.0 cluster_background: true statistics: one-sided hypergeometric scipy.stats.hypergeom.sf(k-1,M,n,N); BH (lib/stats_util.bh_fdr) within scope x level x vocabulary; scope-B fdr_bh_all_species = BH across species tests of one level x vocabulary. goatools only parses the OBO and gives is_a ancestors. candidate_check: matrix_in_rule_and_0_out: 10 candidates_txt: 10 symmetric_difference: 0 candidates_file: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/results/yc_ustilaginomycotina/candidates.txt presence_matrix: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/results/yc_ustilaginomycotina/presence_matrix.uniprot.tsv rule: IN count / 4 >= 0.75 and OUT count / 4 <= 0.0 (own proteome counts toward the target group) only_in_matrix_rule: [] only_in_candidates_file: [] background_clustering: key: 80981bb1d83b4101 n_sequences: 20411 n_clusters: 10919 mmseqs: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/.pixi/envs/default/bin/mmseqs mmseqs_version: 18.8cc5c command: mmseqs easy-cluster --threads N --min-seq-id 0.3 -c 0.8 --cov-mode 0 runtime_s: 39.9 threads: 4 created: '2026-10-24T14:40:02' cache_hit: false pipeline_candidate_families: 'novelties..tsv family_id (nf_NovInvenio MMSEQS_CLUSTER: mmseqs easy-cluster --min-seq-id 0.3 -c 0.8 --cov-mode 0 on candidates.fa)' candidates_without_family_row: 0 go_obo: path: /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/data/go/go-basic.obo data_version: releases/2026-07-26 sha256: b08d45b268b8c24ccb2513dbbbc7d4df9f6521c099b413f79eb31e06e0fa3bcc pfam_dat: path: /srv/projects/db/pfam/2026-01-27-Pfam38.2/Pfam-A.hmm.dat sha256: ead9a40cdb871e1e6ceb151ea15c7aeaff083add2558031b4811afb9d7496b2d release_note: release taken from directory name; the .dat file has no version line interpro_entries: /srv/projects/db/interproscan/6.0.0/110.0/interpro/110.0/entries.json inputs_sha256: ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/config.csv : 3355dc9d4a7f88cd41e5d1aeb5e381d6b1ce69c1d8cd906782cd777822a76666 /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/results/yc_ustilaginomycotina/candidates.txt: bd9e3a5406bcc3e70bb2b0b3913cae769e0f7a89eb414f5a45d061429c3810c1 /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/results/yc_ustilaginomycotina/novelties.Malaglob.tsv: 18c84c5599db6f47bbe3bf62fbcbf90099bc9bdc606aadeea3144980166f28e7 /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/results/yc_ustilaginomycotina/novelties.Malasymp.tsv: 7ef6b3f7a4113a50d7ca81d0cd80c009dbe6aed3dfac2b3fd1a1b49b3992ae46 /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/results/yc_ustilaginomycotina/novelties.Moesaphi.tsv: 64d1bde6917971260dcdb41f902cbbbfa520331eccb930f4fd108bfa1f84c3d7 /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/results/yc_ustilaginomycotina/novelties.Kalmbras.tsv: 64d1bde6917971260dcdb41f902cbbbfa520331eccb930f4fd108bfa1f84c3d7 /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/results/yc_ustilaginomycotina/presence_matrix.uniprot.tsv: b4836f78f90bf631f0c11fec63f8c14acb822c687a4317d50b41462136257959 ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/data_dir/pep/UP000008837_425265.pep.fa : b04ad9cf4378628541da28c9bb394e9f74e7a18c283279106e58214fc840f330 ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/data_dir/pep/UP000186303_1230383.pep.fa : 0f145951fff56d202d835f0224eb3226cd6ec067115f5355ec38697bc7a47266 ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/data_dir/pep/UP000019462_84754.pep.fa : 81bc024265c8f56816b213c2bae43e8f1942948c7c8bb5e91e314c695c95c02b ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/data_dir/pep/UP000019377_1365824.pep.fa : a021c6cde3bc17a85b7f99f9673da8b494dfc0a62c6d2880d39fa8200e0a75ea ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/species.csv : 7d35e52fc3df317fdd89aef0ea8475c8df6d0f80922ca85aafd1dfe295874fbc ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000008837.tsv.gz : 5196b874a4c777d8dfb52e36d6b585c0ed611dd656af948a2c8e80055948a6c6 ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000186303.tsv.gz : df6801222786aa8b1654c08c8c4f11610b1360ba9462484cd50180a09ca8ec2d ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000019462.tsv.gz : 592e5c75c273d3aa45db92cb5e013dfeb848074ec0967e9f79dffd61ea56ada6 ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000019377.tsv.gz : 8b4b208683184402d8ef14192010071a4d8827b20daad7fede522802102279fe ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000000561.tsv.gz : 58da95b04b106212125016a94c470c165934e832f536989fc3976baeddc3ae68 ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000008867.tsv.gz : 3b99bbdc2a40eb72f8a811a469948a2987a38d06e54ba61bfdb46e4773433f4e ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000006174.tsv.gz : f67d31553bc8cc751ef6e56c33fe507f23c39d9e5254555566ee4c3e62855f24 ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/yeast_convergence/yc_ustilaginomycotina/annotations/UP000054845.tsv.gz : bf2d958c6012c6cc10b58ed6ce423943741d8f9d8f80cb92083b41cc1d8b6d2e ? /bigdata/stajichlab/jstajich/projects/NovInvenio_Investigations/studies/fungi/zoosporic_dikarya/analysis/enrichment/actin_controls.tsv : dd7c85c91f413f3eee48b75b51c1ad75f7e2de6afcb611c5e0b3659f777a23d1 tools: python: 3.12.14 scipy: 1.18.1 goatools: 1.6.4 mmseqs: 18.8cc5c runtimes_s: load_s: 4.1 background_cluster_s: 40.1 tests_s: 2.9 total_s: 48.0 host: r11 slurm_job: '30452604'